Estudios científicos
Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy.
Abstract:
Resveratrol is proposed to account in part for the protective effect of red wine on the cardiovascular system. Angiotensin II (Ang II) is a potent hypertrophic stimulus in cardiomyocytes. In this study, we determined the effect of resveratrol on Ang II-induced cardiomyocyte hypertrophy. Cultured neonatal rat cardiomyocytes were stimulated with Ang II, and [3H]leucine incorporation and beta-myosin heavy chain (beta-MyHC) promoter activity were examined. Intracellular reactive oxygen species (ROS) were measured by a redox-sensitive fluorescent dye, 2' 7'-dichlorofluorescin diacetate, and the extracellular signal-regulated kinase (ERK) phosphorylation was examined by Western blotting. Resveratrol inhibited Ang II-increased intracellular ROS levels. Furthermore, resveratrol, as well as the antioxidant N-acetyl-cysteine, decreased Ang II- or H2O2-increased protein synthesis, beta-MyHC promoter activity, and ERK phosphorylation. In summary, we demonstrate for the first time that resveratrol inhibits Ang II-induced cardiomyocyte hypertrophy via attenuation of ROS generation.
Comentarios divulgativos:
El resveratrol explica en parte el efecto protector del vino tinto sobre el sistema cardiovascular. La angiotensina II (Ang II) es un potente estímulo hipertrófico en cardiomiocitos. En este estudio, se determinó el efecto del resveratrol sobre la Ang II y la hipertrofia inducida. Cardiomiocitos de ratas neonatales fueron estimulados con Ang II, y [3H] se midio la incorporación de leucina a la cadena pesada de miosina beta-(beta-MyHC) y las especies reactivas del oxígeno (ROS) se midieron con un tinte fluorescente redox-sensibles, 2 '7'-diacetato dichlorofluorescin, la señal extracelular-quinasa regulada (ERK) fosforilación se analizó por Western Blot. El resveratrol inhibe los niveles intracelulares de Ang II-aumento los niveles de ROS. Por otra parte, el resveratrol, así como el antioxidante N-acetil-cisteína, disminuyeron la Ang II, y la síntesis de proteínas H2O2 aumentó, la actividad beta-MyHC promotor, y la fosforilación de ERK. En resumen, demostramos por primera vez que el resveratrol inhibe la Ang II-inducida por la hipertrofia de cardiomiocitos a través de la atenuación de la generación de ROS.
