Assessment of probiotic properties in lactic acid bacteria isolated from wine
Probiotic properties are highly strain-dependent but rarely studied in enological lactic acid bacteria (LAB). In this study, the probiotic features of 11 strains of Lactobacillus spp., Pediococcus spp., and Oenococcus oeni, including saliva and acid resistance, bile tolerance and exopolysaccharides' production, were investigated. The assays included two probiotic reference strains (Lactobacillus plantarum CLC 17 and Lactobacillus fermentum CECT5716). The Lactobacillus and Pediococcus strains showed high resistance to lysozyme (>80% resistance to 100 mg/L of lysozyme under conditions simulating the in vivo dilution by saliva) and were capable of surviving at low pH values (pH 1.8) and bile salts, suggesting good adaptation of the wine strains to gastrointestinal conditions. The ability of the strains to adhere to the intestinal mucosa and the inhibition of the adhesion of Escherichia coli to human intestinal cells were also evaluated. Adhesion levels of enological LAB to Caco-2 cells varied from 0.37% to 12.2%, depending on the strain. In particular, Pediococcus pentosaceus CIAL-86 showed a high percentage of adhesion to intestinal cells (>12%), even higher than that shown by the probiotic reference strains, and a high anti-adhesion activity against E. coli CIAL-153 (>30%), all of which support this wine LAB strain as a potential probiotic.
Aunque las propiedades probióticas son raramente estudiados en las bacterias del ácido láctico que se encuentran en el vino, este estudio observó las características probióticas de 11 cepas diferentes; resistencia al ácido y saliva, tolerancia a la bilis, entre otros. La capacidad de estas cepas para adherirse a la mucosa intestinal y otras habilidades apoyan al hecho que las bacterias del ácido láctico que se encuentran en el vino son, de hecho, un probiótico potencial.
Even though probiotic properties are rarely studied in lactic acid bacteria found in wine, this study observed the probiotic characteristics of 11 different strains; saliva and acid resistance, bile tolerance, among others. The ability of these strains to adhere to intestinal mucosa and other abilities support that lactic acid bacteria found in wine are in fact a potential probiotic.