Estudios científicos
Combination treatment with resveratrol and sulforaphane induces apoptosis in human U251 glioma cells.
Abstract:
Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.
Comentarios divulgativos:
El resveratrol es un compuesto polifeólico presente en uvas cacahuetes y vino tinto. Estudios previos realizados por este departamento demostraron que el resveratrol cuando se usa en altas concentraciones inhibe la proliferación celular causando la parada del ciclo celular y la inducción de la apoptosis en células de glioma. En el siguiente estudio este departamento estudia la combinación del tratamiento con resveratrol y sulforaphane cuando ambos se usan a bajas concentraciones. Se observa la proliferación celular, la migración y muerte de células humanas U251. Los resultamos muestran que la combinación del tratamiento con ambos compuestos inhiben la proliferación celular y la migración, reducen la viabilidad celular e inducen la liberación de la lactato deshidrogenasa.
