Estudios científicos
Inhibition of human lung cancer cell proliferation and survival by wine
Abstract:
BACKGROUND: Compounds of plant origin and food components have attracted scientific attention for use as agents for cancer prevention and treatment. Wine contains polyphenols that were shown to have anti-cancer and other health benefits. The survival pathways of Akt and extracellular signal-regulated kinase (Erk), and the tumor suppressor p53 are key modulators of cancer cell growth and survival. In this study, we examined the effects of wine on proliferation and survival of human Non-small cell lung cancer (NSCLC) cells and its effects on signaling events.
METHODS: Human NSCLC adenocarcinoma A549 and H1299 cells were used. Cell proliferation was assessed by thymidine incorporation. Clonogenic assays were used to assess cell survival. Immunoblotting was used to examine total and phosphorylated levels of Akt, Erk and p53.
RESULTS: In A549 cells red wine inhibited cell proliferation and reduced clonogenic survival at doses as low as 0.02%. Redwine significantly reduced basal and EGF-stimulated Akt and Erk phosphorylation while it increased the levels of total and phosphorylated p53 (Ser15). Control experiments indicated that the anti-proliferative effects of wine were not mediated by the associated contents of ethanol or the polyphenol resveratrol and were independent of glucose transport into cancer cells. Whitewine also inhibited clonogenic survival, albeit at a higher doses (0.5-2%), and reduced Akt phosphorylation. The effects of both red and white wine on Akt phosphorylation were also verified in H1299 cells.
CONCLUSIONS: Red wine inhibits proliferation of lung cancer cells and blocks clonogenic survival at low concentrations. This is associated with inhibition of basal and EGF-stimulated Akt and Erk signals and enhancement of total and phosphorylated levels of p53. White wine mediates similar effects albeit at higher concentrations. Our data suggest that wine may have considerable anti-tumour and chemoprevention properties in lung cancer and deserves further systematic investigation in animal models of lung cancer.
Comentarios divulgativos:
Este estudio observó que el vino tinto inhibe la proliferación de células de cáncer de pulmón a bajas concentraciones debido a que contiene polifenoles que han demostrado tener propiedades contra el cáncer, así como otros beneficios para la salud. Se observó que el vino blanco tiene el mismo efecto con concentraciones más altas que el vino tinto. Los datos de este estudio sugieren que el vino puede tener propiedades beneficiosas en el cáncer de pulmón y merece una investigación más sistemática.
This study observed that red wine inhibits the proliferation of lung cancer cells at low concentrations due to containing polyphenols that have been shown to have anti-cancer properties as well as other health benefits. White wine was observed to have the same effect at higher concentrations. The data this study obtained suggests that wine may have considerable properties beneficial in lung cancer and deserves further systematic investigation.
