Estudios científicos
Neuroprotective effects of resveratrol against beta-amyloid-induced neurotoxicity in rat hippocampal neurons: involvement of protein kinase C.
Abstract:
1. Resveratrol, an active ingredient of red wine extracts, has been shown to exhibit neuroprotective effects in several experimental models. 2. The present study evaluated the neuroprotective effects of resveratrol against amyloid beta(Abeta)-induced toxicity in cultured rat hippocampal cells and examined the role of the protein kinase C (PKC) pathway in this effect. 3. Pre-, co- and post-treatment with resveratrol significantly attenuated Abeta-induced cell death in a concentration-dependent manner, with a concentration of 25 microm being maximally effective. 4. Pretreatment (1 h) of hippocampal cells with phorbol-12-myristate-13-acetate, a PKC activator, at increasing concentrations (1-100 ng x ml(-1)), resulted in a dose-dependent reduction in Abeta-induced toxicity, whereas the inactive 4alpha-phorbol had no effect. 5. Pretreatment (30 min) of hippocampal cells with GF 109203X (1 microm), a general PKC inhibitor, significantly attenuated the neuroprotective effect of resveratrol against Abeta-induced cell death. 6. Treatment of hippocampal cells with resveratrol (20 microm) also induced the phosphorylation of various isoforms of PKC leading to activation. 7. Taken together, the present results indicate that PKC is involved in the neuroprotective action of resveratrol against Abeta-induced toxicity.
Comentarios divulgativos:
El resveratrol, un ingrediente activo de los extractos del vino tinto, se ha demostrado que presenta efectos neuroprotectores en varios modelos experimentales. El presente estudio evaluó los efectos neuroprotectores del resveratrol en contra de amiloide beta (Abeta) que induce a toxicidad en las células cultivadas del hipocampo de rata y se examinó el papel de la proteína quinasa C (PKC) en este sentido. El Pre-, co-y post-tratamiento con resveratrol Abeta atenuó significativamente la muerte celular inducida de manera dependiente de la concentración, con una concentración siendo la máxima eficacia a 25 micras. El tratamiento previo (30 min) de las células del hipocampo con 109203X GF (1 micras), un inhibidor de la PKC en general, atenuo el efecto neuroprotector del resveratrol contra el Abeta a la muerte celular inducida. El tratamiento de las células del hipocampo con resveratrol (20 micras) también indujo la fosforilación de diversas isoformas de la PKC y condujo a la activación. En conjunto, los resultados indican que la PKC está involucrada en la acción neuroprotectora del resveratrol contra la toxicidad inducida por Abeta.
