Estudios científicos

Procyanidin C1 Inhibits Melanoma Cell Growth by Activating 67-kDa Laminin Receptor Signaling.

Abstract:

Scope: Procyanidin C1 (PC1) is an epicatechin trimer found mainly in grapes that is reported to provide several health benefits. However, little is known about the molecular mechanisms underlying these benefits. The aim of this study is to demonstrate the molecular mechanisms by which PC1 operates.

Methods and results: A 67-kDa laminin receptor (67LR) is identified as a cell surface receptor of PC1, with a Kd value of 2.8 µm. PC1 induces an inhibitory effect on growth, accompanied by dephosphorylation of the C-kinase potentiated protein phosphatase-1 inhibitor protein of 17 kDa (CPI17) and myosin regulatory light chain (MRLC) proteins, followed by actin cytoskeleton remodeling in melanoma cells. These actions are mediated by protein kinase A (PKA) and protein phosphatase 2A (PP2A) activation once PC1 is bound to 67LR.

Conclusion: It is demonstrated that PC1 elicits melanoma cell growth inhibition by activating the 67LR/PKA/PP2A/CPI17/MRLC pathway.

Comentarios divulgativos:

Entre los compuestos polifenólicos, con propiedades bioactivas, presentes en las uvas y en el vino se encuentran las procianidinas. Estos compuestos se han relacionado con propiedades anticancerígenas en estudios in vivo. La procianidina C1 es una de las más abundantes y está formada por tres unidades de epicatequina.

El mecanismo a través del que produce sus efectos no está completamente claro, pero los autores de este estudio plantean que pueda estar relacionado con la activación de una vía de señalización que regula el crecimiento celular y la estructura de la célula (67LR/PKA/PP2A/CPI17/MRLC), tal como sugieren sus resultados.