Estudios científicos
Protective effects of red wine flavonols on 4-hydroxynonenal-induced apoptosis in PC12 cells
Abstract:
There is accumulating evidence that a moderate consumption of red wine has health benefits, such as the inhibition of neurodegenerative diseases. Although this is generally attributed to resveratrol, the protective mechanisms and the active substance(s) remain unclear. We examined whether and how red wine extract (RWE) and red wine flavonols quercetin and myricetin inhibited 4-hydroxynonenal (HNE)-induced apoptosis of rat pheochromocytoma PC12 cells. RWE attenuated HNE-induced PC12 cell death in a dose-dependent manner. HNE induced cleavage of poly(ADP-ribose) polymerase, which is involved in DNA repair in the nucleus, and this was inhibited by RWE treatment. Treatment with RWE also inhibited HNE-induced nuclear condensation in PC12 cells. Data of 2',7'-dichlorofluorescin diacetate showed that RWE protected against apoptosis of PC12 cells by attenuating intracellular reactive oxygen species. The cytoprotective effects on HNE-induced cell death were stronger for quercetin and myricetin than for resveratrol. HNE-induced nuclear condensation was attenuated by quercetin and myricetin. These results suggest that the neuroprotective potential of red wine is attributable to flavonols rather than to resveratrol.
Comentarios divulgativos:
Muchas son las evidencias de que una moderada consumición de vino tinto tiene efectos beneficiosos en la salud como la inhibición de enfermedades neurodegenerativas. Aunque esto es generalmente atribuido al resveratrol muchos aspectos están por esclarecer. En est estudio se examina como extractos de vino tinto y flavonides provenientes del vino tinto como quecertina y miricertina inhiben al compuesto HNE el cual induce la apoptosis en células PC12 en ratas. El efecto protector contra la muerte celular inducida por HNE fue mayor para la quecertina y miricertina que para el resveratrol. Estos resultados sugieren la neuroprotección asociada al vino tinto es debida a los flavonoides más que al reveratrol.
