Estudios científicos
Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cell
Abstract:
The role of 4-hydroxynonenal (4-HNE), a byproduct of membrane lipid peroxidation has been suggested in neurodegeneration. However, the underlying mechanisms are poorly understood. The investigations were carried out to study the preventive potential of trans-resveratrol against 4-HNE induced damage in PC12 cells. Trans-resveratrol, a natural compound obtained from grape skin and found in red wine, is reported to have wide pharmacological window. Cells pretreated with trans-resveratrol (5, 10 and 25 muM) for 24 h were exposed to 4-HNE (25 muM) for 2 h. Pretreatment of trans-resveratrol was found to be significantly effective in countering the cytotoxic responses of 4-HNE. Significant reduction in reactive oxygen species generation, restoration of intracellular glutathione, and lipid peroxidation levels suggest the improved antioxidant defense system in the cells pretreated with trans-resveratrol. Further, 4-HNE induced alterations in the protein expression of mitochondria-mediated apoptosis markers (Bax, Bcl-2 and Caspase-3) were significantly restored by pretreatment of trans-resveratrol suggesting the protective potential of trans-resveratrol in PC12 cells against 4-HNE induced oxidative damage. Together these data show the prophylactic potential of trans-resveratrol in oxidative stress mediated apoptotic neurodegeneration.
Comentarios divulgativos:
El 4-hydroxinonenal (4-NHE) el cual es un producto de la peroxidación lipídica en la membrana se ha sugerido que puede estar implicado en la neurodegeneración. Sin embargo los mecanismos a través de los cuales actuán aún no están muy claros. Este estudio indaga en el posible potencial preventivo del resveratrol o trans-resveratrol contra el 4-HNE en el daño celular en células PC12. Células pretratadas con diferentes concentraciones de resveratrol durante 24 horas fueron expuestas a 4-HNE durante 2 horas. Se observó que el pretratamiento con resveratrol fue significativamente efectivo en contener la respuesta citotóxica provocada por el 4-HNE. Se encontaró una reducción en las especies reactivas de oxigeno y restauración de los niveles de peroxidación lipídica lo cual demuestra la defensa antioxidante que el resveratrol ocasiona.
