Red wine and oenological extracts display antimicrobial effects in an oral bacteria biofilm model
The antimicrobial effects of red wine and its inherent components on oral microbiota were studied by using a 5-speciesbiofilm model of the supragingival plaque that includes Actinomyces oris, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus mutans and Veillonella dispar. Microbiological analysis (CFU counting and confocal laser scanning microscopy) of the biofilms after the application of red wine, dealcoholized red wine, and red wine extract solutions spiked or not with grape seed and inactive dry yeast extracts showed that the solutions spiked with seed extract were effective against F. nucleatum, S. oralis and A. oris. Also, red wine and dealcoholized wine had an antimicrobial effect against F. nucleatum and S. oralis. Additional experiments showed almost complete and early degradation of flavan-3-ol precursors [(+)-catechin and procyanidin B2] when incubating biofilms with the red wine extract. To our knowledge, this is the first study of antimicrobial properties of wine in an oral biofilm model.
Se estudiaron los efectos antimicrobianos que puede tener el vino tinto y sus componentes sobre los microbios que se pueden encontrar en la boca utilizando vino tino, vino tino desalcoholizado y soluciones de extracto de vino tinto con y sin semilla de uva y extractos de levadura seca inactiva. Las soluciones que contienen extracto de semilla fueron eficaces con tres microbios diferentes (F nucleatum, S orali and A oris), mientras que el vino tino y el vino desalcoholizado tuvieron un efecto antimicrobiano contra dos (F nucleatum y S oralis).
The antimicrobial effects of red wine and its components on microbes that can be found in the mouth were studied using red wine, dealcoholized red wine and red wine extract solutions with and without grape seed and inactive dry yeast extracts. The solutions containing seed extract were effective against three different microbes (F nucleatum, S. oralis and A. oris), while the red wine and dealcoholized wine had an antimicrobial effect against two (F nucleatum and S oralis).