Estudios científicos

Resveratrol, a polyphenolic compound in red wine, protects against oxidized LDL-induced cytotoxicity in endothelial cells.

Abstract:

BACKGROUND: Resveratrol, a polyphenolic constituent of red wine, has antioxidant effects. However, its protective effects against oxLDL-induced endothelial injury remained unclarified.
METHODS: Primary human umbilical vein endothelial cell cultures (HUVECs) treated with oxLDL (200 microg/ml) were used to explore the protective effect of resveratrol. Cytotoxicity of oxLDL on HUVECs was studied by measuring lactate dehydrogenase (LDH) release, methylthiazol tetrazolium (MTT) and apoptotic cell death as characterized by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) stain. We also measured the production of reactive oxygen species (ROS) by using the fluorescnet probe 2', 7'-dichlorofluorescein acetoxymethyl ester (DCF-AM), and observed the activity of antioxidant enzymes. Furthermore, several apoptotic signaling pathway with increased cytosolic calcium, alteration of mitochondrial membrane potential, cytochrome c release and activation of caspase 3 were also investigated.
RESULTS: Resveratrol attenuated oxLDL-induced cytotoxicity, apoptotic features, generation of ROS and intracellular calcium accumulation. OxLDL-induced mitochondria membrane potential collapase, cytochrome c release and activation of caspase 3 in HUVECs were also suppressed by resveratrol pretreatment.
CONCLUSIONS: Red wine intake may protect against oxLDL-induced dysfunction of endothelial cells.
 

Comentarios divulgativos:

El resveratrol, un componente polifenólico del vino tinto, tiene efectos antioxidantes. Sin embargo, sus efectos protectores contra la lesión endotelial inducida por LDLox están sin aclarar.
Cultivos de células endoteliales de vena umbilical (HUVEC) tratados con LDLox (200 microg / ml) se utilizan para explorar el efecto protector del resveratrol. La citotoxicidad de LDLox en HUVECs se estudió mediante la medición de lactato deshidrogenasa (LDH), y la muerte celular por apoptosis, caracterizado porel marcaje terminal deoxynucleotidyl final dUTP nick-transferasa- (TUNEL) También se evaluó la producción de especies reactivas del oxígeno (ROS) utilizando la sonda fluorescnet 2 ', 7'-éster acetoxymethyl diclorofluoresceína (DCF-AM), y se observó la actividad de las enzimas antioxidantes. Además, fueron investigadas varias vías de señalización apoptótica con el aumento del calcio citosólico, la alteración del potencial de membrana mitocondrial, liberación del citocromo c y la activación de la caspasa 3.
El resveratrol atenua la citotoxicidad de LDLox por las características de la apoptosis, la generación de ROS y la acumulación de calcio intracelular.
El consumo de vino tinto puede proteger contra la disfunción LDLox inducida por las células endoteliales.