Estudios científicos
Resveratrol inhibits foam cell formation via NADPH oxidase 1- mediated reactive oxygen species and monocyte chemotactic protein-1
Abstract:
Resveratrol is a polyphenolic compound in red wine that has anti-oxidant and cardioprotective effects in animal models. Reactive oxygen species (ROS) and monocyte chemotactic protein-1 (MCP-1) play key roles in foam cell formation and atherosclerosis. We studied LPS-mediated foam cell formation and the effect of resveratrol. Resveratrol pretreatment strongly suppressed LPS-induced foam cell formation. To determine if resveratrol affected the expression of genes that control ROS generation in macrophages, NADPH oxidase 1 (Nox1) was measured. Resveratrol treatment of macrophages inhibited LPS-induced Nox1 expression as well as ROS generation, and also suppressed LPS-induced MCP-1 mRNA and protein expression. We investigated the upstream targets of Nox1 and MCP-1 expression and found that Akt-forkhead transcription factors of the O class (FoxO3a) is an important signaling pathway that regulates both genes. These inhibitory effects of resveratrol on Nox1 expression and MCP-1 production may target to the Akt and FoxO3a signaling pathways.
Comentarios divulgativos:
El resveratrol es un compuesto polifenólico presente en el vino tinto el cual tiene efectos antioxidantes y cardioprotectores en modelos animales. Las especies reactivas de oxigeno y la proteina 1 (MCP-1) juegan un papel importante en la formación de la espuma de aislamiento celular y la arterosclerosis.En este estudio se investiga sobre la formación de la espuma de aislamiento celular mediada por LPS y el efecto que tienen el resveratrol sobre este mecanismo. Para determinar si el resveratrol afecta a la expresión de los genes que controlan la generación de ROS en los macrófagos se midió la NADPH oxidasa (Nox1). Los efectos inhibitorios del resveratrol en la expresión de Nox1 y MCP-1 pueden ser la diana de las rutas de Akt y FoxO3a.
