Estudios científicos
Resveratrol inhibits prostaglandin formation in IL-1beta-stimulated SK-N-SH neuronal cells
Abstract:
Resveratrol, a polyphenol present in grapes and red wine, has been studied due to its vast pharmacological activity. It has been demonstrated that resveratrol inhibits production of inflammatory mediators in different in vitro and in vivo models. Our group recently demonstrated that resveratrol reduced the production of prostaglandin (PG) E2 and 8-isoprostane in rat activated microglia. In a microglial-neuronal coculture, resveratrol reduced neuronal death induced by activated microglia. However, less is known about its direct roles in neurons. In the present study, we investigated the effects of resveratrol on interleukin (IL)-1beta stimulated SK-N-SH cells. Resveratrol (0.1-5 microM) did not reduce the expression of cyclooxygenase (COX)-2 and microsomal PGE2 synthase-1 (mPGES-1), although it drastically reduced PGE2 and PGD2 content in IL-1beta-stimulated SK-N-SH cells. This effect was due, in part, to a reduction in COX enzymatic activity, mainly COX-2, at lower doses of resveratrol. The production of 8-iso-PGF2alpha, a marker of cellular free radical generation, was significantly reduced by resveratrol. The present work provides evidence that resveratrol reduces the formation of prostaglandins in neuroblastoma cells by reducing the enzymatic activity of inducible enzymes, such as COX-2, and not the transcription of the PG synthases, as demonstrated elsewhere.
Comentarios divulgativos:
El resveratrol es un polifenol presente en uvas y vino tinto el cual ha sido estudiado según su actividad farmacologica. Se ha demostrado que el resveratrol inhibe la producción de mediadores inflamatorios en modelos in vivo e in vivo. En estudios anteriores este grupo ha demostrado que el resveratrol reduce la producción de prostaglandinas (PG) E2 y 8-isoprostane en ratas con la microglia activada. En un cultivo de neuronas y microglia el resveratrol reduce la muerte neuronal inducida por la activación de la microglia, sin embargo se conoce menos sobre el papel directo en neuronas. En el siguiente estudio estudiamos los efectos del resveratrol en la interleukina (IL)-1 beta estimulada en células SK-N-SH. Los autores concluyen que el resveratrol reduce la formación de prostaglandinas en células del neuroblastoma reduciendo la actividad enzimatica de enzimas como COX-.2
