Estudios científicos

Resveratrol regulates human adipocyte number and function in a Sirt1-dependent manner


BACKGROUND: Caloric restriction leads to retardation of the aging processes and to longer life in many organisms. This effect of caloric restriction can be mimicked by resveratrol, a natural plant product present in grapes and red wine, which is known as a potent activator of sirtuin 1 [silent mating type information regulation 2 homolog 1 (Sirt1)]. OBJECTIVES: One main effect of caloric restriction in mammals is a reduction of body fat from white adipose tissue. We sought to identify the effects of resveratrol on fat cell biology and to elucidate whether Sirt1 is involved in resveratrol-mediated changes. DESIGN: Human Simpson-Golabi-Behmel syndrome preadipocytes and adipocytes were used to study proliferation, adipogenic differentiation, glucose uptake, de novo lipogenesis, and adipokine secretion. Sirt1-deficient human preadipocytes were generated by using a lentiviral small hairpin RNA system to study the role of Sirt1 in resveratrol-mediated changes. RESULTS: Resveratrol inhibited preadipocyte proliferation and adipogenic differentiation in a Sirt1-dependent manner. In human adipocytes, resveratrol stimulated basal and insulin-stimulated glucose uptake. De novo lipogenesis was inhibited in parallel with a down-regulation of lipogenic gene expression. Furthermore, resveratrol down-regulated the expression and secretion of interleukin-6 and interleukin-8. Sirt1 was only partially responsible for the regulation of resveratrol-mediated changes in adipokine secretion. CONCLUSIONS: Taken together, our data suggest that resveratrol influences adipose tissue mass and function in a way that may positively interfere with the development of obesity-related comorbidities. Thus, our findings open up the new perspective that resveratrol-induced intracellular pathways could be a target for prevention or treatment of obesity-associated endocrine and metabolic adverse effects.

Comentarios divulgativos:

La restricción calórica es lo que guía el envejecimiento en muchos organismos. Este efecto de restricción calórica puede ser disminuido gracias al resveratrol, este compuesto químico esta presente en derivados de plantas como las uvas y el vino tinto y es un potente activador del compuesto sirtuin1. El objetivo de este estudio es identificar los efectos del resveratrol en el metabolismo de las grasas de la célular e identificar como el compuesto Sirtuin-1 esta relacionado en estos cambios a través del resveratrol.
Los hallazgos de este estudio abren la puerta a que el resveratrol induce rutas intracelulares que pueden ser la diana para tratamientos en la prevención de la obesidad asociada a efectos metabólicos y endocrinos.