Estudios científicos

Suppression of type-I allergic responses by oral administration of grape marc fermented with Lactobacillus plantarum


We investigated the inhibitory effects of fermented grape marc (FGM), lyophilized fine powder of skin, and seeds of Vitis vinifera Koshu grape prepared by fermentation with Lactobacillus plantarum, on type-I allergic responses in mice. Repeated oral administration of FGM, but not non-fermented grape marc (GM), to BALB/c mice primed with ovalbumin (OVA) resulted in a significant reduction of serum IgE levels, compared with those of immunized controls. After OVA challenge, increased numbers of eosinophils in bronchial alveolar lavage fluids (BALF) significantly decreased by treatment with FGM but not with GM. For passive cutaneous anaphylaxis (PCA) reaction, BALB/c mice received intradermal sensitization with anti-OVA IgE serum and were challenged intravenously with OVA containing Evans blue at 24 h after IgE sensitization. Oral administration of FGM at 30 min before OVA challenge significantly suppressed the PCA reaction. On the other hand, Lactobacillus alone and non-fermented GM did not show any suppressive effects. Interestingly, FGM samples prepared from grapes for red wine, such as Negroamaro (rich in resveratrol) or Tannat (rich in oligomeric procyanidin), did not suppress the reaction. These results indicate that oral administration of FGM, prepared from Koshu grape for white wine but not from grapes for red wine, could suppress both phases of type-I allergic responses. A fraction extractable with acetone was responsible for the suppressive effects of FGM.

Comentarios divulgativos:

En este estudio se investiga los efectos inhibitorios de la huella de uvas fermentadas (FMG), el polvo liofilizado de la piel y las semillas de Vitis vinifera Koshu preparadas con fermentación de Lactobacillus plantarum en la respuesta alérgica tipo I en ratones.
Los resultados muestran que la administración de de FMG preparado de Koshu de vino blanco, que no de vino tinto, pueden suprimir ambas fases de la respuesta alérgica de tipo I. La fracción extraíble con acetona es la responsable de los efectos supresores.