The janus face of resveratrol in astroglial cells
Astroglial cells are key modulators of neuropathology events. Resveratrol, a redox-active compound present in grapes and wine, has a wide range of biological effects. The aim of this study was to investigate whether resveratrol is able to prevent hydrogen peroxide (H(2)O(2))-induced oxidative damage in C6 astroglial cells. We found that following a short oxidative insult (Model I-1 mM H(2)O(2)/30 min), resveratrol increased glutamate uptake (60%), glutamine synthetase (GS) (139%), glutathione (GSH) (120%), and S100B secretion (24%); and attenuated DCFH oxidation (34%) as compared to H(2)O(2) values. Under less intense (0.1 mM H(2)O(2)), but lasting (6 h) insult (Model II), resveratrol had an opposite effect, potentiating the H(2)O(2)-induced decrease in glutamate uptake (from 34 to 63%), in GS (from 22 to 50%), in GSH (from 22 to 54%), and also potentiating DCFH oxidation (from 24 to 38%). The transcription factor, NF-kappaB, was activated in both models. Cell morphology alterations were also observed in the presence of H(2)O(2) with process-bearing cells, accompanied by cell body retraction and actin reorganization. This effect was not prevented by resveratrol, but was prevented by lysophosphatidic acid (LPA), a specific upstream positive regulator of Rho A. In summary, these findings showed that resveratrol, a redox-active compound, was able to modulate important neurotrophic function of astroglial cells under different oxidative conditions.
Las células astrogliares son factores claves en los eventos neuropatológicos las cuales ectuan como moduladores. El proposito de este estudio es investigar como el resveratrol es capaz de prevenir la formación de peroxido de hodrógeno induciendo el daño celular en las células C6 astrogliares. Se ha observado que el resveratrol incrementa la toma de glutamato (60%), glutamina sintetasa (GS 139%) glutation (GSH 120%) y la secreción de S100B (24%) y atenua la oxidación de DCFH (34%) cuando lo comparamos con los valores de H2O2. En resumen estos hallazgos muestran que el resveratrol es capaz de regular funciones neurotróficas importantes dependiendo de diferentes condiciones oxidativas.