Estudios científicos
The red wine polyphenol resveratrol reduces polycyclic aromatic hydrocarbon-induced DNA damage in MCF-10A cells
Abstract:
Polycyclic aromatic hydrocarbons (PAH) are procarcinogens that can be commonly found in our food and environment. Upon biotransformation in our body system, they can cause DNA damage through the generation of genotoxic species and oxidative stress. Phase I and II enzymes are pivotal in the process of proximate carcinogen formation and elimination. Some dietary phytochemicals are strong inhibitors to the phase I enzymes. In the present study, we investigated the effect of the red wine compound resveratrol on DNA damage induced by PAH in a non-tumorigenic breast cell line MCF-10A. Resveratrol ranging from 1 to 5 microm could significantly suppress the expressions of cytochrome P450 (CYP) 1A1, CYP1B1 and UDP-glucuronosyltransferase (UGT) 1A1 induced by 7,12-dimethylbenz[a]anthracene (DMBA). The comet assay indicated that DMBA introduced DNA damage to these cells, and co-treatment of resveratrol at 5 or 10 microm could alleviate the damage. Further investigation illustrated that resveratrol reduced the binding of DMBA metabolites to DNA with no effect on DMBA-induced oxidative DNA damage. Since the phase II enzyme UGT1A1 was suppressed, the elimination of DMBA metabolites would not have contributed to the reduction in the DMBA metabolite-DNA binding. In summary, resveratrol might protect breast cells against PAH-induced DNA damage. The underlying mechanism was mediated by phase I enzyme suppression rather than phase II enzyme induction or oxidative DNA repair.
Comentarios divulgativos:
Los hidrocarburos policiclicos aromaticos (PAH) son carcinogénicos y pueden ser encontrados en nuestra comida y en el medio ambiente. Estos compuestos pueden causar daño en el DNA a traves de la generación de compuestos genotóxicos y estress oxidativo. Enzimas de clase I y clase II están implicadas en el proceso de formación carcinogénica, algunos compuestos fitoquímicos son fuertes inhibidores de las enzimas de fase I. En el siguiente estudio se investigan los efectos del resveratrol del vino tinto en el daño del DNA inducido por PAH en lineas celulares MDF-10A. El resveratrol puede proteger las células del cancer de pecho contra PAH. El mecanismo de acción esta mediado principalmente por la supresión de enzimas de clase I.
